I plan to ask about how to restore some defunct human genes. The GULO pseudo gene is the first one I am going to ask about. Should the question maybe be split into several question? The intended question is as follows.
A lot of base pairs in the defunct GULO pseudo gene in humans are missing to make a functional enzyme.
Should we take a whole gene from a close relative like a strepsirrhine, or maybe just fill in what is missing based on what?
Which cells should be transfected/infected and how?
Where should the working gene be put in the genome? Should the defunct gene be kept or replaced?
Transcription levels for the GULO pseudo gene are too low to make enough enzymes to increase ascorbate levels above levels achievable by consumption. How can that be increased?
I am not asking if this is beneficial or not nor about regulatory or ethical questions. Such questions are also meaningful but should be discussed in other questions.